Follow this innovation
[ via 
] [?]
Innovation
CHO-K1 Cell Lines Expressing the Human EP3A, EP3D, EP3E, and EP3F Prostanoid Receptors
University of Arizona
posted on 10/19/2009
Title:
CHO-K1 Cell Lines Expressing Prostanoid Receptors
Basis of
Invention: University of Arizona researchers have created chinese hamster ovary
cells expressing the human EP3A, EP3D, EP3E and EP3F prostanoid receptors. These
G-coupled receptors are widely found in smooth muscle such as the
gastrointestinal tract and in bronchial tissue. They are proposed to mediate
contractile activity of the smooth muscle. As such, the new cell lines are
expected to be a useful research tool for investigating receptor activity: they
can be used in functional studies of receptor activation and second messenger
signaling. They will also provide a sensitive and reliable measure of the
potential for drugs to interact with the receptor.
The
Technology: Chinese hamster ovary cell lines were transformed with plasmids
containing each of the alternative mRNA splice variants of the prostanoid
receptors. The plasmid conferred neomycin resistance on the cells, allowing
selection of transformed cells. Transformation was confirmed using a variety of
standard techniques.
Highlights:
* Stably
transformed cell lines
* Useful
in research into receptor activity and signaling
* Sensitive
and reliable measure of the potential for drugs to interact with the receptors.
Related UA
technologies: UA04-052
State of
Development. Have stable cell lines expressing the receptors.
Innovation Details
File Number: UA04-053
IP Protection
License Online
This innovation currently is not available for online licensing. Please contact Suzanne Dubuque at University of Arizona for more information.
request more info
ProfileFind more innovations
