Mouse Anti-Histone Auto-Antibody (clone F152C25W)

University of Missouri System: University of Missouri-Columbia
posted on 06/17/2011

• request more info 
•   
•  LICENSE ONLINE

Histones are the chief organizing proteins of DNA in the nucleus of all eukaryotic cells. They can be grouped into five major classes, whereof four form a nucleosome core particle that DNA is wrapped around, and the fifth organizes the linker DNA between the nucleosomes. Histones are among the most highly conserved proteins between species, which underscores their essential function for life of eukaryotic cells.

The current invention developed by researchers at University of Missouri is a mouse monoclonal antibody from an autoimmune mouse model that detects all five classes of histones. The antibody reacts with several vertebrate species, including human, bovine, mouse, hamster, and marsupials, and has been shown to work well with Western blots, indirect immunofluorescence, and ELISA.

• Detection of histones by Western and ELISA
  
• Staining of cells using immunofluorescence

• Hybridoma cell line available
  
• Easy to produce in quantities
  
• Detects all classes of histones
  
• Can be used with multiple vertebrate species
  
• Can be used with a variety of applications
  
• IgG2a isotype allows for easy affinity purification