Pharmacologic Enhancement of Bacteriophage-Mediated Gene Transfer and Plasmid DNA-Mediated Gene Transfer in Mammalian Cells

University of Rochester
posted on 07/01/2009

Suggested Uses

This technology proposes enhancing both bacteriophage-mediated gene transfer and plasmid DNA-mediated gene transfer in mammalian cells using pharmacological agents. This is applicable for vaccines as well as other gene transfer needs and as a research tool in the laboratory.

Advantages

These methods are advantageous as they enhance the gene activity achieved with bacteriopage-mediated or plasmid DNA-mediated delivery alone. More efficient gene transfer is possible by co-deliver agents that inhibit the proteosome or endosome acidification and by disrupting cellular microtubules. Additionally this may permit stronger immune responses and greater protein expression.

Detailed Description

This technology proposes enhancing both bacteriophage-mediated gene transfer and plasmid DNA-mediated gene transfer in mammalian cells using pharmacological agents. This is applicable for vaccines as well as other gene transfer needs and as a research tool in the laboratory.

These methods are advantageous as they enhance the gene activity achieved with bacteriopage-mediated or plasmid DNA-mediated delivery alone. More efficient gene transfer is possible by co-deliver agents that inhibit the proteosome or endosome acidification and by disrupting cellular microtubules. Additionally this may permit stronger immune responses and greater protein expression.

File Number: 6-1629

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