Cockroach allergens are known to be major contributors to the incidence of asthma. These allergens are primary derived from decayed fecal pellets, body parts, shed cuticles and egg cases, as well as secretions, which later become aerosolized and settle as dust. Unfortunately, cockroach allergens are almost indefinitely stable and remain long after the infestation has been cleared.

Certain stable molecular species were found to be associated with dust samples containing cockroach allergens. More importantly, the concentration of these molecules was also found to be directly proportional to the total allergen level in dust samples. Simple reliable methods were developed that assay for these molecules as a measure of total cockroach allergen levels, in order to ascertain the severity of the contamination.

One method uses a simple test kit for use on location, and provides a quick, rough estimate of the total allergen level. This method involves contacting a liquid suspension of the dust sample with a strip of dry impregnated filter paper. The intensity of the color that develops is directly proportional to the total allergen level in the sample. Other methods use an inexpensive colorimeter or fluorimeter, routinely available in most testing laboratories, and provides a more precise quantitative determination of the total allergen level.

The cost of goods for a single test is estimated at just a few cents. Due to the low cost and high sensitivity, these methods have the potential to greatly facilitate allergen abatement, which is essential for reducing the incidence of asthma.

File Number: 438FC 

Web site: http://www.temple.edu/ott