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Polynucleotide Ligands As Antiviral Agents
University of California System: University of California, Berkeley
posted on 05/28/2009
Infectious human cytomegalovirus (HCMV) were isolated in vitro from a pool of randomized sequences after sixteen or 21 cycles of selection and amplification. The ligands characterized exhibited high HCMV-binding affinity in vitro and effectively inhibited viral infection in tissue culture. Several ligands blocked viral entry. Their antiviral activity was also specific as the ligands only reacted with strains of HCMV, but not with the related herpes simplex virus 1 and human cells. Moreover, the ligands recognize several different epitopes. Thus, RNA ligands can function to bind to a human virus and block viral infection. The screening method may utilize the novel features of binding to intact infectious virus, partitioning the bound polynucleotides from unbound by passing through a porous filter, and enhancing the release of bound polynucleotides by treatment with protease.
Suggested Uses
Antiviral agent
Research tool to identify viral proteins required for infectivity.
Advantages
Inhibits human cytomegalovirus infection.
File Number: 16882
| Patent Number(s): | WO0138341, 6849610 |
|---|---|
| Copyright: | ©2009-2010, The Regents of the University of California |
This innovation currently is not available for online licensing. Please contact Javed Afzal at University of California System: University of California, Berkeley for more information.
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