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Micro-patterned Photoliable Surfaces for Capture and Light Triggered Release of Cells
University of California System: University of California, Davis - UC Davis
posted on 12/14/2011
Surfaces are frequently micropatterned with proteins in order to capture and culture cells in distinct gerometric configurations. Researchers at UC Davice have developed a novel method for micropatterning surfaces with photoliabile protein to capture and release of cells, triggered by UV light.
Suggested Uses
This technology is a useful as a research tool to capture specific groups of cells or individuals cells from micropatterned surface. The micropatterned surfaces also have important application in cell based diagnostics, tissue engineering and developmental biology.
Advantages
- The micropatterned surface contains target specific binding sites.
- The method is compatible with standard available
- The method is compatible with standard microscopes available in most research labs.
- The method does not compromise cell viability.
Detailed Description
The researchers at UC Davis have developed a novel method for micropatterning surfaces with photoliabile protein to capture and release of cells, triggered by UV light. In this approach, surfaces were modified using a mixture of amine- and acrylate-terminated methoxysilanes and then micropatterned using poly (ethylene glycol)(PEG) photolithography. There surface were further functionalized with photolabile linker containing photosensitive o-nitrobenzyl group. Finally, the surfaces were functionalized with specific antibodies. This multi-step surface modification protocol resulted in formation of microwells with non-fouling walls and antibody containing cell capture sites created on glass via photo-cleavable molecules. The studies show that a short exposure to UV was sufficient to cleave anchors holding cells to the surface, leading to cell release upon gentle agitation. Importantly, this cell retrieval protocol did no compromise to cell viability.
File Number: 22194
| Copyright: | ©2011, The Regents of the University of California |
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This innovation currently is not available for online licensing. Please contact Madhu Sharma at University of California System: University of California, Davis - UC Davis for more information.
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