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Regulated Overexpression of NH3 Driven by its own Promoter enhances Disease Resistance in Plants
University of California System: University of California, Davis - UC Davis
posted on 07/30/2010
NPR1 (non-expresser of pathogenesis related genes 1) is the master regulator of salicyclic acid-mediated systemic acquired resistance. Over-expression of Arabidopsis NPR1 and rice NH1 (NPR1 homolog1)/OsNPR1 in rice results in enhanced resistance. Researchers at UC Davis investigated a rice NPR1 paralog for similar activity. Regulated overexpression of NH3, when driven by its own promoter (nNT-NH3), resulted in clear, enhanced resistance when challenged with Xanthomonas oryzae pv. oryzae.
Suggested Uses
NH3 has the potential to engineer a broad spectrum of resistance (oomyces, bacteria and viruses).
Advantages
Constitutively activating defense responses not only wastes energy and resources but often also leads to undesirable consequences, such as dwarf plants. This invention represents a novel, effective approach to enhance disease resistance without constitutively activating plant defense responses and thus offers many advantages over existing methods.
This invention will make the use of chemical inducers more effective and hence reduce the amount of chemicals needed to achieve desired yields.
Inventors
- Bai, Wei
- Chern, Mawsheng
- Ronald, Pamela C.
Detailed Description
NH3 has never been shown to enhance disease resistance. However, introduction of an extra copy of NH3 driven by its own promoter resulted in a confirmed enhanced resistance phenotype, as measured by Xoo-induced lesion length. Bacterial growth curve analysis indicates that bacterial population levels are reduced 10-fold in nNT-NH3 lines compared to control rice lines. The transgenic plants exhibit higher sensitivity to benzothiadiazole and INA treatment as measured by increased cell death. Further, the transgenic plants display greatly enhanced induction of pathogen resistance genes (up to 244-fold) only after treatment with BTH.
File Number: 21021
| Patent Number(s): | WO2011150117 |
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| Copyright: | ©2010-2012, The Regents of the University of California |
This innovation currently is not available for online licensing. Please contact Randi Jenkins at University of California System: University of California, Davis - UC Davis for more information.
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