A Novel Technique for Fabricating Biomolecular Nano-Arrays Enabling High-DNA Amplification and Sequencing
University of California System: University of California, San Diego
posted on 07/01/2009
Brief description unavailable
This technology may be applied to high-throughput genome sequencing, protein array analysis, and bio-sensors.
This invention provides a new approach for fabricating high-density nano-arrays for bio-molecules. Researchers used glass slides derivatized with functional groups, then coated them with a thin layer of photo-resist. Conventional photolithography is then used to create high-density wells of sub-micron dimension into which nano-particles conjugated to a single DNA clone are allowed to self-assemble. The small size of the wells prevents more than one molecule from attaching in any given well. The nano-particles remain fixed in their wells through biotin-avidin with the remaining non-specifically bound nano-particles being washed away with the removal of the remaining photo-resist. This leaves a very clean array for high throughput sequencing.
File Number: 19180
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