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The Chip-Dasl Technology For Functional Genomics Studies
University of California System: University of California, San Diego Technology Transfer Office
posted on 07/01/2009
Transcriptional regulation involves a large number of protein complexes specifically assembled at a given promoter to activate or suppress RNA synthesis. In a specific tissue or cell type, a promoter can be turned on by a sequence of specific recognition events. Transcription factors bind cis-acting regulatory sequences and allow these DNA binding proteins to recruit co-activator complexes that further recruit the core transcription machinery. Similarly, a gene can be turned off by the recruitment of transcription co-repressor complexes through sequence-specific DNA binding proteins during repression-involved chromatin remodeling factors that modify histones. A long-term molecular memory may be established by epigenetic modification of a specific chromatin region(s) via DNA methylation.
Detailed Description
Scientists at UC San Diego have developed a new method, which advances the understanding of DNA binding proteins and allows functional genomics questions to be addressed under a variety of physiological conditions. This method allows selective enrichment of functional DNA elements, such as promoters/enhancers, replication origins, and specifically modified sequences, within any genome of interest followed by signal amplification and detection. This is accomplished by coupling the selective enrichment of the functional DNA elements (by chromatin immunoprecipitation, ChIP) with a signal amplification/detection procedure, (DNA Annealing Selection and Ligation, or DASL) which utilizes microarrays. This novel technology, called ChIP-DASL permits robust analysis using fewer materials compared with to standard procedures. The invention has broad applications in the fields of basic research (gene expression studies), clinical diagnosis, and high throughput pharmaco-genomics screening (drug screening assays).
File Number: 19173
Other Information:
Patent Information
The patent application numbers are US 200710059703 and WO 20051007814.
| Patent Number(s): | 7601492 |
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| Copyright: | ©2009-2010, The Regents of the University of California |
This innovation currently is not available for online licensing. Please contact University of California, San Diego Technology Transfer Office at University of California System: University of California, San Diego Technology Transfer Office for more information.
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