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Non-Covalent Chemical Reprogramming Of Cellular Adhesion with Membrane Anchored Nucleic Acids
University of California System: University of California, San Francisco
posted on 11/30/2011
Cell adhesion is an essential function that mediates the physical interaction betweeen cells and their microenvironment and plays an important role in tissue formation. Chemical control of cell adhesion allows for temporal and spatial manipulations of cell-cell and cell-surface interactions with high resolution for therapeutic and research purposes. Recent reports show that cell-surface grafted nucleic acids can serve as adhesion molecules that have the benefits of minimal cross reactivity with endogenous cell-surface receptors and combinatorial encoding of interactions. Cell surfaces can be modified with DNA either covalently or non-covalently through direct linkage of oligonucleotides to hydrophobic molecules such as lipids and steroids. Current labeling approaches have several disadvantages, including manufacturing difficulties, inability to stably integrate into the cell surface under typical culture conditions, interfering with cellular function, and failure to display adhesive sequence at controlled distances from the cell surface.
Suggested Uses
- Cell/Tissue engineering
- Stem cell research
- High throughput screening of non-adherent cells
- Live cell imaging
- Cell-based therapies
- Regenerative medicine
- Wound healing
- Laboratory/research reagents and kits
- Bio-adhesives
- Cell-cell fusion/hybridoma development
Advantages
- Allows for efficient reprogramming of cell adhesion for both adherent and non-adherent cells
- Covalent modification of cells not required
- Does not affect cell viability
- Cells proliferate and behave identically to unmodified cells
- Easily synthesized from commercially available chemicals
- Has defined chemical composition that does not activate cell receptors
- Works in all metazoan cells
Detailed Description
UCSF investigators have developed a novel strategy for non-covalently labeling cell membranes with lipid-oligonucleotide conjugates that have improved performace when compared to state-of-the-art chemical adhesion molecules. They have successfully demonstrated rapid, efficient, and tunable labeling of cell surfaces with these unique conjugates: a platform methodology resulting in cells of high viability and enabling capture and adhesion to precise locations on complementary labeled surfaces such as glass substrates, or self-assembly into 3D microtissues. The UCSF researches have also showed that this method can be utilized to image non-adherent cells on inert surfaces over extended periods.
File Number: 22140
| Copyright: | ©2011, The Regents of the University of California |
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This innovation currently is not available for online licensing. Please contact Ellen Kats at University of California System: University of California, San Francisco for more information.
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