This hybridoma produces monoclonal antibodies to the Fc portion of the murine IgD haplotype Igh-Ca. The antibodies are IgG2b, recognize mouse Igh-Ca, and have been demonstrated in flow cytometry, immunofluorescence and stimulation of B-cell proliferation.
Suggested Uses
Flow cytometry, immunofluorescence and stimulation of B-cell proliferation
Detailed Description
IgD is the major antigen receptor isotype coexpressed with IgM on the surface of most peripheral B cells.(Note: No cross-reactivity with haplotypes Igh-Cb or Igh-Ce has been observed for this antibody.)
Hybridoma Production:
SJL mice were immunized with spleen cells from BALB/c mice and the resultant SJL splenocytes were fused with SP2/0 cells. Recognition of IgD haplotype Igh-Ca was confirmed by FACS analysis.
References:
Stall, A. and M. Loken (1984) "Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies" J Immunol 132(2):787
Goroff, D., A. Stall, J. Mond and F. Finkelman (1986) "In vitro and in vivo B lymphocyte-activating properties if monoclonal anti-d antibodies. I. Determinants of B-lymphocyte-activating properties" J Immunol 136:2382