Hybridoma Producing Monoclonal Antibodies to Mouse IgM (Igh-Cb and related haplotypes)

University of Chicago
posted on 10/20/2009

Hybridoma AF6-78.25.4 produces monoclonal antibodies that recognize the murine IgM haplotype Igh-Cb and related haplotypes. The antibodies recognize mouse Igh-Cb and have been demonstrated to work for flow cytometry, immunofluorescence and ELISA assays.

Suggested Uses

Flow cytometry, Immunofluorescence, ELISA

Innovation Details

Detailed Description

IgM is the first antibody class produced during a humoral response. IgM is also co-expressed with IgD on the surface of B-cells. Antibody Class – Mouse IgG1 Species recognized – Mouse Hybridoma Production: BALB/c mice were immunized with spleen cells from C57BL/10 mice and the resultant BALB/c splenocytes were fused with SP2/0 cells. Recognition of IgD haplotype Igh-Ca was confirmed by FACS analysis. References: Stall, A. and M. Loken (1984) “Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies” J Immunol 132(2):787

File Number: 1123

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