Mouse H2-M3 MHC Class Ib Monoclonal Antibody

University of Chicago
posted on 08/18/2009

Hybridoma mAb 130 (Murine H2-M3 MHC Class Ib) UCHI 0838 Antigen: Mouse H2-M3 MHC Class Ib Antigen Background: H2-M3 MHC class Ib is a non-classical MHC class I molecule that is specialized for binding N -formyl peptides. N -formyl peptides are produced by prokaryotes during protein synthesis, but are rare in mammalian cells. H2-M3 restricted CD8+ T-cells are early responders during primary infections, appearing 1-2 days earlier than MHC class Ia restricted memory CTLs. H2-M3 plays a significant role in responding to bacterial infections and appears to be important in bridging the innate and adaptive immune responses. Antibody Class: Hamster IgG H2-M3 species recognized: Mouse Hybridoma Production: The soluble portion of H2-M3 was purified from D. melanogaster cell line SC2 and used to immunize Armenian hamsters. Lymphocytes isolated from immunized hamsters were used to produce hybridoma cell lines by fusion with murine myeloma cell line SP2/0. Hybridoma supernatants were screened for H2-M3 recognition by ELISA and recognition was confirmed by the ability to block the recognition of H2-M3-restricted CTLs.

Suggested Uses

Demonstrated Uses: Immunofluorescence, flow cytometry, ELISA, functional blocking of T cells and immunoprecipitation

Innovation Details

File Number: 0838

Other Information:

References:

Chiu et al. 1999. J. Exp. Med. 190:423

Lybarger et al. 2001. J. Immunol. 167:2097

Wang et al. 2005. J. Virol. 79:4099

Xu et al. 2006. J. Exp. Med. 203:449

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