Monoclonal Antibody Hybridoma to Ovalbumin

University of Chicago
posted on 08/18/2009

Hybridoma F2-3.58 (murine anti-OVA) Antigen Ovalbumin, the main protein found in chicken egg white. Antigen Background phosphoprotein constituent of egg white Antibody Class mouse IgG1 Species recognized chicken Demonstrated Uses � ELISA assay Hybridoma Production: Three Balb/cj female mice were immunized intraperitoneally on day 0, 14, and 21 with 100 ug OVA in Complete or Incomplete Freund�s Adjuvant. Splenocytes from one mouse were fused with SP2/0-Ag14 myeloma cells on approximately day 65. Clones were screened on OVA-coated ELISA plates and positives subcloned by limiting dilution. References: No publications. This antibody was produced to serve as a positive control for use in the University of Chicago Frank W. Fitch Monoclonal Facility.

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File Number: 1844

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