Five Prime Binding and Isolation of Pol II RNA

University of Utah Technology Commercialization Office
posted on 10/26/2011

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Early Work: Purifying mRNAs with a high-affinity eIF4E mutant identifies the short 3' poly(A) end phenotype - Biochemistry - 2003

"procedure for the purification of eukaryotic mRNAs using a mutant version of the mRNA 5' cap-binding protein (eIF4E) with increased affinity for the m7GTP moiety of the cap"

Research Applications:

Purification and analysis of 5' capped RNAs; related to RNA origin, mRNAs, miRNAs and RNAs with short poly(A) ends

Example analysis of 5' capped RNA provides more information from biospecimens

New disclosures and technologies are available for review under confidentiality

Example Published Applications:

Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs - 2004

A novel method for poly(A) fractionation reveals a large population of mRNAs with a short poly(A) tail in mammalian cells - 2007

Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors - 2010

Genome-wide characterization of methylguanosinecapped and polyadenylated small RNAs in the rice blast fungus Magnaporthe oryzae - 2010

ENCODE Tiling Array Analysis Identifies Differentially Expressed Annotated and Novel 59 Capped RNAs in Hepatitis C Infected Liver - 2011

Intellectual Property: US Patents 6,841,363 and 6,841,363 are issued

US 6,596,854 Independent Claims - An isolated nucleic acid molecule encoding a variant human eIF4E protein having altered binding affinity for m7G-RNA compared to natural human eIF4E protein, wherein said variant protein has an amino acid substitution in the region of amino acids 112 and 114-121