Full-Length cDNA Libraries from Dioxin- and Vehicle-Treated Mice

Wisconsin Alumni Research Foundation (University of Wisconsin)
posted on 06/23/2009

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing full-length cDNA libraries isolated from non-parenchymal mouse liver cells.

Suggested Uses

Improve our understanding of dioxin’s mechanism of toxicity
Determine which genes are responsive to dioxin

Advantages

Both libraries contain full-length clones and were isolated at the same time.
Provides— for the first time—a snap shot of the effects of dioxin on gene expression in mammals
Provides potential targets for the development of dioxin treatments
May enable the development of screens for dioxin in the environment

Innovation Details

Detailed Description

Dioxin is a highly toxic environmental pollutant that acts primarily through the aryl hydrocarbon receptor. Exposure to dioxin has adverse effects on the immune, nervous, endocrine and reproductive systems, and has been shown to cause cancer in animals. To better understand the effects of this toxin and develop ways of treating dioxin contamination, scientists need to discover which genes are responsive to dioxin.

UW-Madison researchers have isolated two full-length cDNA libraries from non-parenchymal mouse liver cells. One library was isolated from dioxin-treated mice and the other from a control group of vehicle-treated mice. These libraries could be used to determine which genes are upregulated and which are downregulated by dioxin.

File Number: P06227US

IP Protection

License Online

This innovation currently is not available for online licensing. Please contact Emily Bauer at Wisconsin Alumni Research Foundation (University of Wisconsin) for more information.

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