’Background:

ATPase-binding cassette transporters (ABC transporters) are transmembrane proteins that use ATP to shuttle molecules across cellular membranes. ABCA1 belongs to the ABC transporter family and functions to transport intracellular phospholipids and cholesterol onto high density lipoproteins in plasma and in the brain. Loss-of-function mutations in ABCA1 give rise to Tangier’s disease where disease hallmarks include accumulation of cholesterol in the lymphatics and low levels of high density lipoproteins (HDL) and HDL-associated lipoproteins.

The current technology describes a hybridoma cell line that produces monoclonal antibodies (mAbs), isotype IgG2b, against murine ABCA1 transporter protein. Purified mAbs of isotype IgG2 are also available for license. ABCA1 mAbs detect the N-terminal extracellular loop of the ABCA1 protein and react with human and rodent ABCA1. Antibodies can be used for western blot analysis with superior results over other commercially available antibodies.

File Number: CU0266