Follow this innovation
[ via 
] [?]
p-neo-LoxP Plasmid
Washington University in St. Louis
posted on 01/03/2007
We are offering a cloning vector containing a pgk-neo cassette flanked by 2 LoxP sites. When used in conjunction with the cre-recombinase, the vector allows for the removal of the pgk-neo cassette.
Suggested Uses
When used in conjunction with the cre-recombinase, the vector allows for the removal of the pgk-neo cassette.
Advantages
This vector allows for easy cloning of targeted arms into the floxed pgk-neo containing vector and eventual removal of the selectable marker cassette, which eliminates possible expression effects on neighboring genes.
Detailed Description
The current technology describes a cloning vector containing a pgk-neo cassette flanked by 2 LoxP sites. The vector also contains a phosphoglycerate kinase promoter and a bovine growth hormone polyadenylation signal. When used in conjunction with the cre-recombinase, the vector allows for the removal of the pgk-neo cassette.
Advantage:This vector allows for easy cloning of targeted arms into the floxed pgk-neo containing vector and eventual removal of the selectable marker cassette, which eliminates possible expression effects on neighboring genes.
Publications:Cell 1991; 64:693-702.
PNAS 1996; 93:13090.
Mol Cell Biol. 1996; 16:2906.
Genbank Accession #: AF335419; For additional p-neo-LoxP plasmid 1339, please see Genbank Accession # AF335420.
File Number: CJ0058
This innovation currently is not available for online licensing. Please contact Jon Kratochvil at Washington University in St. Louis for more information.
request more info
ProfileFind more innovations
