p-neo-LoxP Plasmid

Washington University in St. Louis
posted on 01/03/2007

We are offering a cloning vector containing a pgk-neo cassette flanked by 2 LoxP sites. When used in conjunction with the cre-recombinase, the vector allows for the removal of the pgk-neo cassette.

Suggested Uses

When used in conjunction with the cre-recombinase, the vector allows for the removal of the pgk-neo cassette.

Advantages

This vector allows for easy cloning of targeted arms into the floxed pgk-neo containing vector and eventual removal of the selectable marker cassette, which eliminates possible expression effects on neighboring genes.

Innovation Details

Detailed Description

Technology Description:The current technology describes a cloning vector containing a pgk-neo cassette flanked by 2 LoxP sites. The vector also contains a phosphoglycerate kinase promoter and a bovine growth hormone polyadenylation signal. When used in conjunction with the cre-recombinase, the vector allows for the removal of the pgk-neo cassette.

Advantage:This vector allows for easy cloning of targeted arms into the floxed pgk-neo containing vector and eventual removal of the selectable marker cassette, which eliminates possible expression effects on neighboring genes.

Publications:Cell 1991; 64:693-702.
PNAS 1996; 93:13090.
Mol Cell Biol. 1996; 16:2906.

Genbank Accession #: AF335419; For additional p-neo-LoxP plasmid 1339, please see Genbank Accession # AF335420.

File Number: CJ0058

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License Online

This innovation currently is not available for online licensing. Please contact Jon Kratochvil at Washington University in St. Louis for more information.

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